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KMID : 1140120080130030177
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Cancer Prevention Research
2008 Volume.13 No. 3 p.177 ~ p.183
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Molecular Mechanism of Progesterone-Induced Apoptosis in Human Breast Cancer T47D Cells
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Im Ji-Young
Lee Byung-Mu
Kwack Seung-Jun
Lee Young-Jun
Kim In-Young
Myung Chang-Sun
Kim Hyung-Sik
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Abstract
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Progesterone plays an essential role in the development and differentiation of the mammary gland. Although it is well established that progesterone regulates cellular proliferation and differentiation, the molecular mechanisms of progesterone-mediated anticancer effects remain unclear. Therefore, the aim of the present study was to determine how progesterone regulates the growth and viability of T47D human breast cancer cells. In this study, the proliferation of T47D cells was inhibited after 48 hours of incubation in the presence of 10μM progesterone. The progesterone-treated T47D cells also exhibited hallmarks of apoptosis including DNA fragmentation and nuclear morphological changes detected with DAPI staining. Also, PR-A and PR-B progesterone receptor expression was decreased in a dose-dependent manner. Moreover, progesterone significantly decreased the expression of p53, cyclin D1, and CDK4, while it increased the expression of p27. These results clearly demonstrate high doses of progesterone induce T47D human breast cancer cell apoptosis and suppress proliferation by inhibiting the expression of key cell cycle regulators. (Cancer Prev Res 13, 177-183, 2008)
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KEYWORD
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Progesterone, Apoptosis, T47D cells, p53, Cyclin D1
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